미니돼지에서 Acellular dermal matrix에 배양된 치주인대섬유세포을 이용한 자가치아이식술: 치주인대로써의 잠재력에 대한 조직학적 평가
Autotransplantation using the acellular dermal matrix seeded by periodontal Ligament fibroblasts in minipig: histological evaluation as potential periodontal Ligament substitutes
유상준, 박주철, 김병옥, 장현선,
소속 상세정보
유상준 ( Yu Sang-Joun ) - 소속확인중
박주철 ( Park Joo-Cheol ) - 소속확인중
김병옥 ( Kim Byung-Ok ) - 조선대학교 치과대학 치주과학교실
장현선 ( Jang Hyun-Seon ) - 소속확인중
KMID : 0363020070370010053
Abstract
The aim of this study was to examine the possibility of periodontal ligament regeneration when autotransplantation was used by the periodontal ligament fibroblasts cultured on the acellular dermal matrix in teeth without a periodontal ligament. One minipig was used in this study. The mandibular and maxillary permanent incisors were ex-tracted for the culture of the periodontal ligament cells. The roots of the unextracted teeth were classified into a positive control group, in which the normal periodontal ligament was preserved. The roots of the extracted teeth were divided into the following two groups: The negative control group, in which the periodontal ligament had been removed and the acellular dermal matrix was not applied; and an experimental group, in which the periodontal ligament had been removed and periodontal ligament fibroblast cultured on an acellular dermal matrix was applied. The prepared teeth were transplanted, and completely submerged using physical barrier membranes. The animal was sacrificed 4 weeks after the autotransplant. The transplanted teeth were examined histologically. In this study, the periodontal ligament was normal in the positive control group, and ankylosis was discovered on the denuded root surface in the negative control group. Periodontal ligament-like connective tissue was found adjacent to the denuded root and the new cementum-like layer of hard tissue was formed in the experimental group. These results suggest that the periodontal ligament fibroblasts cultured on the acellular dermal matrix may play a role in regenerating the periodontal ligament-like tissue with new cememtum-like tissue formation.
키워드
autotransplantation;periodontal Ligament;cell-seeding;acellular dermal matrix
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